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Product Info Summary
| SKU: | M00061 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Hu CD195 Purified CCR5 Monoclonal Antibody
SKU/Catalog Number
M00061
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD195 Purified CCR5 Monoclonal Antibody (Catalog# M00061). Tested in Flow Cytometry, IP, WB, ICC, ELISA application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD195 Purified CCR5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00061)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
T21/8
Isotype
Mouse IgG1 kappa
Immunogen
CCR5 peptide (Met1-Lys22) KLH conjugate. The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00061 is reactive to CCR5 in Human
Applications
M00061 is guaranteed for ELISA, Flow Cytometry, IP, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
40.524kDa
Background of CCR5
CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: Do not boil cell lysates prior to SDS-PAGE.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of CCR5 using Anti-Hu CD195 Purified CCR5 Monoclonal Antibody (M00061).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD195 (T21/8) purified antibody (concentration in sample 3 μg/ml) GAM APC.
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Figure 2. Flow Cytometry validation of CCR5 using Anti-Hu CD195 Purified CCR5 Monoclonal Antibody (M00061).
Separation of human CD195 positive lymphocytes (red-filled) from CD195 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD195 (T21/8) purified antibody (concentration in sample 3 μg/ml) GAM APC.Protein Target Info & Infographic
Gene/Protein Information For CCR5 (Source: Uniprot.org, NCBI)
Gene Name
CCR5
Full Name
C-C chemokine receptor type 5
Weight
40.524kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5 CCR5 CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22 C-C motif chemokine receptor 5 C-C chemokine receptor type 5|C-C motif chemokine receptor 5 A159A|HIV-1 fusion coreceptor|chemokine (C-C motif) receptor 5|chemokine receptor CCR5|chemokine recptor CCR5 Delta32|chemr13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR5, check out the CCR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD195 Purified CCR5 Monoclonal Antibody (M00061)
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No publications found for M00061
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